产品参数

产品概述

Cell Meter 细胞增殖检测试剂盒是一种灵敏的荧光法检测试剂盒，以微板形式量化细胞并评估细胞增殖和细胞毒性。该试剂盒使用我们的Nuclear Green LCS1，一种专有的荧光染料，在与核酸结合时表现出强烈的荧光。核绿色LCS1的使用提高了细胞代谢、细胞增殖或细胞毒性试验的准确性，这些细胞增殖或细胞毒性试验可能受到与细胞数量无关的细胞变化的影响。该试剂盒不需要细胞裂解、长时间孵育或去除细胞，它与高通量筛选兼容。

适用仪器

实验方案

样品实验方案

概述

准备细胞样本，并根据需要处理细胞

去除细胞培养基

添加染料工作溶液

培养10分钟

使用Ex/Em=490/525 nm的荧光酶标仪进行分析

重要提示：在打开瓶子之前，将所有成分加热到室温。由于该试剂与核酸结合，应将其作为潜在的诱变剂处理，并适当谨慎处理。DMSO储备液应特别小心处理，因为已知DMSO有利于有机分子进入组织。

溶液配制

1.工作溶液配制

Nuclear Green LCS1工作溶液配制
向10 m L反应缓冲液中加入25 µL的Nuclear Green LCS1，并充分混匀。

注：10 mL 足以进行100次试验。为了获得效果，Nuclear Green LCS1染料工作液应及时使用。

注：在- 20 ° C保存任何未使用的Nuclear Green LCS1 (成分A )。

操作步骤

以下方案仅用于指导，具体，请按照自己的实验情况进行调整。

1.制备细胞样本并按需处理细胞。

2.去除细胞培养基，将细胞用PBS清洗2次。
注意：去除细胞培养基是必须的，因为它可能干扰Nuclear Green LCS1的荧光。

3.可选：用PBS洗涤后，涉及多个时间点和样品的实验可以在- 80 ° C保存几天或进行步骤4。

4.加入100 µ L的Nuclear Green LCS1染料工作液，室温孵育10 ~ 60 min，避光。

5.使用Ex / Em = 490 / 525 nm ( Cutoff = 515 nm)的荧光酶标仪检测样品荧光。 

图示

图1.使用Cell Meter 细胞增殖检测试剂盒对HeLa细胞进行定量。将细胞的线性范围从50到50，000 HeLa细胞铺板，并按照方案进行检测。

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